21st Annual US HUPO Conference
February 22 - 26, 2025 | Philadelphia, PA, USA
AFFINISEP BREAKFAST SEMINAR
Innovative SPE Kits for Sample Preparation in MS-Based Proteomics
Tuesday, Febuary 25th, 2025 | 7:15 AM - 8:15 AM
Tuesday, Febuary 25th, 2025 | 7:15 AM - 8:15 AM
Innovative SPE Kits for Sample Preparation in MS-Based Proteomics: From Peptide Desalting to Glycopeptide Enrichment, Our Customers Say It Best!
During this breakfast seminar, we will have the pleasure to welcome Dr. Aaron Storey, Director of the Mass Spectrometry lab at Arkana Laboratories, and Dr. Parastoo Azadi, Associate Director of the Complex Carbohydrate Research Center at the University of Georgia, both convinced users of our BioSPEĀ® kits.
First, Dr. Aaron Storey will present his work on the implementation of a membranous nephropathy typing assay on a triple quadrupole mass spectrometer in clinical practice, and will describe how the use of the BioSPEĀ® PurePep 96 well plates for microelution helped him minimize variances during the desalting step of his protocol. By attending his talk, you will learn that:
Assays for measuring autoantibody levels in serum are needed by the nephrology community
A workflow for immune complex extraction and typing membranous nephropathy in frozen tissue by MRM has been developed
Determining the protein targeted by the immune system is important for etiology, prognosis, and treatment
Membranous nephropathy is caused by immune complex deposition in kidney tissue and driven by autoantibodies
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Affinisep develops and manufactures from A to Z high quality and ready-to-use microelution BioSPEĀ® kits to simplify sample preparation in proteomics workflows and help you achieve highly reliable results. Our wide range of formats and capacities allow us to cover a broad range of needs, from a few samples processed manually, to high throughput processing of hundreds of samples in clinical studies.
Then, Dr. Parastoo Azadi will talk about cutting-edge strategies for the enrichment and structural characterization of glycoproteins and will present the advantages of using BioSPEĀ® GlycaClean spin columns for glycopeptide enrichment. Her presentation will focus on the following points:
Challenges in analysis of glycoproteins from cells or tissues
Release strategies for glycoproteins
Enrichment methods for glycoproteins/glycoproteomics
Enhanced MS fragmentation strategies for glycosidic and ring cleavages of derivatized glycans
Why we need to pay attention to non-carbohydrate constituent of glycans?
ā¶ Aaron Storey, Ph.D.
Dr. Aaron Storey joined Arkana Laboratories in 2023 to direct the Mass Spectrometry Laboratory and is interested in developing and applying mass spectrometry techniques in clinical settings to improve the practice of medicine and address clinical needs. His academic career has provided him with a broad knowledge base, spanning molecular biology, neuroscience, genetics, proteomics, statistics, and data science. His skill set combines the application of cutting-edge proteomics techniques to generate large data sets containing high quality information, and the use of programming languages and data analysis techniques to turn this information into actionable insights. Currently, Dr. Storey is working on translating a mass spectrometry-based assay for typing membranous nephropathy into clinical practice. Dr. Storey received his B.Sc. in Behavioral Neuroscience in 2010 from the University of Evansville and his Ph.D. in Biochemistry & Molecular Biology in 2017 from the University of Arkansas for Medical Sciences.
ā· Parastoo Azadi, Ph.D.
Dr. Parastoo Azadi is the Associate Director at the Complex Carbohydrate Research Center and the PI on the NIH National Glycoscience Resource & the DOE Center for Plant and Microbial Complex Carbohydrates. She also heads the service and training facility at the CCRC where she performs services for academic, government, non-profit organizations and private companies, throughout the United States and internationally. She works closely with scientists in industry on developing carbohydrate-based drugs and the need for out-sourcing prior to phase I and phase II clinical trials. Dr. Azadiās laboratory also develops cutting edge techniques & methodologies for structural characterization of plant, bacterial and animal polysaccharides, glycoproteins and glycolipids using MS and NMR techniques. Dr. Azadi received her B.Sc. in Chemistry in 1987 from University of North London, UK and her Ph.D. degree in Biochemistry in 1991 from Imperial College of Science and Technology, University of London, studying structural characterization of carbohydrates and glycoproteins by mass spectrometry.